The detergent fibre analysis scheme was introduced to overcome inadequacies in the use of the traditional acid–alkali crude fibre estimation when applied to fibrous forage feeds for ruminants (Van Soest, 1970; see table).
The determination of ADF involves the extraction of food (1 g) by boiling (1 h) in aciddetergent solution (100 ml; 2% cetyltrimethylammonium bromide (CTAB) in 0.5 M H2 SO4 ). The insoluble residue is filtered, washed with acetone, dried (8 h, 100°C) and weighed. This residue, which includes cellulose, lignin and some inorganic elements such as silica, is described as ADF. The residue can be used for subsequent measurement of cellulose after oxidation of lignin by saturated potassium permanganate solution and removal of manganese dioxide by oxalic acid (Van Soest and Wine, 1968).
The determination of ADF involves the extraction of food (1 g) by boiling (1 h) in aciddetergent solution (100 ml; 2% cetyltrimethylammonium bromide (CTAB) in 0.5 M H2 SO4 ). The insoluble residue is filtered, washed with acetone, dried (8 h, 100°C) and weighed. This residue, which includes cellulose, lignin and some inorganic elements such as silica, is described as ADF. The residue can be used for subsequent measurement of cellulose after oxidation of lignin by saturated potassium permanganate solution and removal of manganese dioxide by oxalic acid (Van Soest and Wine, 1968).
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